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When added to a growing chain, dNTPs prevent DNA synthesis because they lack a hydroxyl group in the 3' position.
What is Sanger sequencing?
The "chain termination method," often known as Sanger sequencing, is a technique for figuring out the nucleotide sequence of DNA. The Sanger Sequence is a technique that was created in 1977 by two-time Nobel laureate Frederick Sanger and his colleagues.
The Sanger sequencing process consists of three key phases.
DNA SEQUENCE FOR ENDING CHAIN PCR
Chain-termination PCR is a unique form of PCR that uses the DNA sequence of interest as a template. While chain-termination PCR functions similarly to normal PCR, it differs significantly in that modified nucleotides (dNTPs) known as dideoxy ribonucleotides are added (ddNTPs).
ELECTROPHORESIS OF GEL TO SEPARATE SIZE
The second stage involves separating the chain-terminated oligonucleotides by size using gel electrophoresis. The oligonucleotides will be drawn toward the positive electrode on the other side of the gel in gel electrophoresis because DNA is negatively charged. DNA samples are placed into one end of a gel matrix and an electric current is applied.
DNA SEQUENCE DETERMINATION & GEL ANALYSIS
The final step consists of simply reading the gel to ascertain the input DNA sequence. Each terminal ddNTP will match a specific nucleotide in the original sequence as DNA polymerase only creates DNA in the 5' to 3' direction beginning at a supplied primer.
Hence, When added to a growing chain, dNTPs prevent DNA synthesis because they lack a hydroxyl group in the 3' position.
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