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If your group is assigned to prepare 60 ml of 1.2% agarose gel solution for part B, what materials are required to prepare the gel solution. Select the amount of agarose and the amount of the correct buffer needed. 0.72 g of agarose 60 ml of electrophoresis buffer with pH at 8.6 60 ml of electrophoresis buffer with pH at 9.2 0.5 g of agarose 59.28 ml of electrophoresis buffer with pH at 9.2

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Agarose gels are prepared using a w/v percentage solution.

Materials required to prepare gel solution and perform electrophoresis are :

1. An electrophoresis chamber and power supply.

2. Gel casting trays, are available in a variety of sizes and are composed of UV-transparent plastic.

3. Sample combs, around which molten agarose is poured to form sample wells in the gel.

4. Electrophoresis buffer, usually Tris-acetate-EDTA (TAE) or Tris-borate-EDTA (TBE).

5. Loading buffer, which contains something dense (e.g. glycerol) to allow the sample to "fall" into the sample wells, and one or two tracking dyes, which migrate in the gel and allow visual monitoring or how far the electrophoresis has proceeded.

6. Ethidium bromide, a fluorescent dye used for staining nucleic acids.

7. Transilluminator (an ultraviolet light box), which is used to visualize ethidium bromide-stained DNA in gels.

- 0.72 g of agarose

- 60 ml of electrophoresis buffer with pH at 9.2

1.2 x 60 / 100 = 0.72

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