Keeping integrase (IN) concentration constant and measuring the initial velocity of the reaction at increasing vDNA concentrations in the presence and absence of the ODN, is called competitive inhibition.
This experimental mechanism is competitive inhibition, where ODN (oligodeoxynucleotides) acts as competitive inhibitors. That is the ODN binds to the integrase instead of vDNA (viral DNA) binding to the enzyme integrase.
To determine the activity of the ODN, the Km and Vmax values should be calculated. Km (Michaelis constant) which shows the affinity of the enzyme to the substrate and Vmax (maximum velocity at saturating substrate concentration).
Calculating these two values in the presence and absence of the inhibitor can determine the activity of the inhibitor (ODN).
Learn more about Competitive inhibition on
https://brainly.com/question/14944549
#SPJ1
