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To transform bacteria with plasmids, technicians first make the bacteria competent (capable of taking up DNA) by placing them in calcium chloride and chilling them. Plasmid is then added to the competent bacteria and the plasmid/bacteria combination is taken through a few more steps to make the bacteria take up DNA. In your experiment, should you treat a tube of bacteria that you do not add plasmid to exactly as you do the tube of bacteria that you will transform: