Using a good compound light microscope with a resolving power of 0.3 µm, a 10x ocular lens, and a 100x oil immersion lens, would you be able to discern two objects separated by 3 µm? 0.3 µm? 300 nm? why would, or wouldn't, you be able to do this with the presented microscope?

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30-06-2018
Chemistry

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Using a good compound light microscope with a resolving power of 0.3 µm, a 10x ocular lens, and a 100x oil immersion lens, would you be able to discern two objects separated by 3 µm? 0.3 µm? 300 nm? why would, or wouldn't, you be able to do this with the presented microscope?

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PBCHEM PBCHEM · Answer 1 · 2018-07-09T13:40:21+02:00

Answer : One would be able to easily discern between two objects as they would be separated by all 4 distances as given in the question; because each one will be greater than or equal to the the resolving power of the compound light microscope.

Resolution means the separation between the two close images so, if we use a microscope with 0.3 µm, a 10x ocular lens, and a 100x oil immersion lens it will be able to resolve images which are greater of the given values.